National Status and Trends, Benthic Surveillance Project DNA-Xenobiotic Adducts Data, 1991, National Centers for Coastal Ocean Science

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Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator:
Department of Commerce (DOC), National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA)
Publication_Date: 1992
Title:
National Status and Trends, Benthic Surveillance Project DNA-Xenobiotic Adducts Data, 1991, National Centers for Coastal Ocean Science
Geospatial_Data_Presentation_Form: table
Publication_Information:
Publication_Place: Silver Spring, MD
Publisher:
NOAA's Ocean Service, National Centers for Coastal Ocean Science (NCCOS)
Online_Linkage: <http://NSandT.noaa.gov>
Description:
Abstract:
In order to determine the current status of and to detect any long-term trends in the environmental quality of U.S. nearshore waters, NOAA initiated the National Status and Trends program in 1984 with its National Benthic Surveillance Project. The primary objective of the Benthic Surveillance Project was to quantify concentrations of a suite of organic and inorganic contaminants in the livers of fish and surficial sediments from selected sites in the coastal and estuarine waters of the United States.In addition, the levels of certain indicators of the biological effects of these contaminants were measured. Incidences of visible lesions, including fin erosion, have been noted and histopathological examinations of various tissues have been carried out. Originally histopathological examinations determined the prevalence of any identifiable disease conditions in samples of liver, kidney, and gill tissue.In addition, the Benthic Surveillance Project tested fish livers from 30 locations in 1991 to identify the concentrations of DNA xenobiotic adducts. DNA xenobiotic adducts are a bioindicator of exposure to genotoxic compounds. Measurement of biological markers such as DNA xenobiotic adducts have been used to improve the evaluation of contaminant exposure and responses in indigenous fish, and thus reinforce the connection between exposure to toxic chemicals and the observed injuries.
Purpose:
In response to concerns over environmental quality of the Nation's coastal and estuarine ecosystems, NOAA created the National Status and Trends (NSandT) Program in 1984. From 1984 through 1993, the Benthic Surveillance Project monitored chemical concentrations in the livers (and for metabolites of PAH's in the bile) of bottom-dwelling fish and in sediments at the sites of fish capture. The Benthic Surveillance Project also measured the biological effects of contaminant exposure, primarily as prevalence's of toxicopathic liver diseases.
Time_Period_of_Content:
Time_Period_Information:
Single_Date/Time:
Calendar_Date: 1991
Currentness_Reference: publication date
Status:
Progress: Complete
Maintenance_and_Update_Frequency: None planned
Spatial_Domain:
Bounding_Coordinates:
West_Bounding_Coordinate: -166.5
East_Bounding_Coordinate: -67.3
North_Bounding_Coordinate: 70.5
South_Bounding_Coordinate: 25.6
Keywords:
Theme:
Theme_Keyword_Thesaurus: CoRIS Discovery Thesaurus
Theme_Keyword: Numeric Data Sets > Chemical Contaminants
Theme:
Theme_Keyword_Thesaurus: ISO 19115 Topic Category
Theme_Keyword: environment
Theme_Keyword: 007
Theme_Keyword: oceans
Theme_Keyword: 014
Theme:
Theme_Keyword_Thesaurus: CoRIS Theme Thesaurus
Theme_Keyword:
EARTH SCIENCE > Human Dimensions > Environmental Impacts > Contaminants
Theme:
Theme_Keyword_Thesaurus: None
Theme_Keyword: DNA
Theme_Keyword: deoxyribonucleic acid
Theme_Keyword: xenobiotic deoxyribonucleic acid adducts
Theme_Keyword: DNA xenobiotic adducts
Theme_Keyword: DNA adducts
Theme_Keyword: DNA abnormalities
Theme_Keyword: genotoxic exposure
Theme_Keyword: Coastal Monitoring
Theme_Keyword: oceans
Theme_Keyword: NSandT
Theme_Keyword: NS&T
Place:
Place_Keyword_Thesaurus: None
Place_Keyword: U.S. Exclusive Economic Zone
Place_Keyword: Baltimore Harbor, Brewerton Channel
Place_Keyword: Boston Harbor, Deer Island
Place_Keyword: Cape Elizabeth, Richmond Island
Place_Keyword: Cape Fear River, Horseshoe Shaol
Place_Keyword: Charlotte Harbor, Cape Haze
Place_Keyword: Chesapeake Bay, Chester River
Place_Keyword: Chesapeake Bay, James River
Place_Keyword: Chesapeake Bay, Patuxent River
Place_Keyword: Choctawhatchee Bay, Choctawhatchee Bay
Place_Keyword: Dana Point Harbor, Outside
Place_Keyword: Delaware Bay, Cherry Island Range
Place_Keyword: Johns Bay, Pemaquid Neck
Place_Keyword: Lower Laguna Madre, Laguna Heights
Place_Keyword: Lower Laguna Madre, Long Island
Place_Keyword: New Bedford Harbor, Clarks Point
Place_Keyword: Pascagoula River, Escatawpa River
Place_Keyword: Pensacola Bay, Pensacola Bay
Place_Keyword: Raritan Bay, East Reach
Place_Keyword: Raritan Bay, Upper Bay
Place_Keyword: Salem Harbor, Folger Point
Place_Keyword: San Antonio Bay, Mosquito Point
Place_Keyword: San Diego Bay, North
Place_Keyword: San Diego Bay, Twenty Eighth Street
Place_Keyword: San Francisco Bay, Hunters Point
Place_Keyword: San Francisco Bay, Southampton Shoal
Place_Keyword: San Pedro Bay, Outer Harbor
Place_Keyword: Sapelo Sound, Dog Hammock
Place_Keyword: St. Andrews Bay, Military Point
Place_Keyword: St. Johns River, Arlington Channel
Place_Keyword: St. Lucie River, Stuart
Place:
Place_Keyword_Thesaurus: CoRIS Place Thesaurus
Place_Keyword: OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida
Place_Keyword: COUNTRY/TERRITORY > United States of America > Florida
Place_Keyword: OCEAN BASIN > Atlantic Ocean
Place_Keyword: OCEAN BASIN > Pacific Ocean
Place:
Place_Keyword_Thesaurus: CoRIS Region
Place_Keyword: Florida
Access_Constraints: None
Use_Constraints:
NOAA requests that all individuals who download NSandT data acknowledge the source of these data in any reports, papers, or presentations. If you publish these data, please include a statement similar to: "Some or all of the data described in this article were produced by NOAA through its National Status and Trends Program".
Point_of_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization:
National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA)
Contact_Position:
National Status and Trends, Benthic Surveillance Project Manager
Contact_Address:
Address_Type: mailing and physical address
Address: 1305 East West Highway N/SCI1
City: Silver Spring
State_or_Province: MD
Postal_Code: 20910
Contact_Voice_Telephone: 301-713-3028
Contact_Facsimile_Telephone: 301-713-4388
Native_Data_Set_Environment:
Microsoft Windows 2000 Version 5.0 (Build 2195) Service Pack 3; ESRI ArcCatalog 8.3.0.800

Data_Quality_Information:
Logical_Consistency_Report:
The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples and analyzing standard reference materials. To assess the reproducibility of results every tissue sample was analyzed in duplicate.
Completeness_Report:
The quality of the analytical data generated by the NSandT Program is overseen by the QA Project component, which has been in operation since 1985 and is designed to document sampling and analytical procedures, and to reduce intralaboratory and interlaboratory variation. The QA Project documentation will facilitate comparisons between different monitoring programs with similar QA activities and thus will extend the temporal and spatial scale of such programs. To document laboratory expertise, the QA Project requires all NSandT laboratories to participate in a continuing series of intercomparison exercises utilizing a variety of materials. The organic analytical intercomparison exercises are coordinated by the NIST, and the inorganic exercises by the National Research Council (NRC) of Canada. Details of quality assurance for the Benthic Surveillance Program can be found in Lauenstein and Cantillo, 1993.
Positional_Accuracy:
Horizontal_Positional_Accuracy:
Horizontal_Positional_Accuracy_Report:
The same kind of field/site data have been supplied since the start of the Benthic Surveillance Project, in 1984. Original site coordinates were derived from Loran-C time conversions. Early sites information resulting from Loran-C was converted from time delay information to latitudes and longitudes. These earlier data may be suspect when sites were located close to large structures that could have interfered with accurate time delays. When GPS was first available the signal was intentionally degrades so earlier coordinate information, even if it resulted from GPS, is not as accurate as data would be today.Because fish are not sessile, fish trawls have been made along different tracks in the water body of interest. The latitude/longitude coordinates provided in this file represent a nominal site center and trawling occurs within a 1 km radius of this location.
Lineage:
Process_Step:
Process_Description:
The primary collection apparatus was Otter trawls. Occasionally, fish were taken with hook and line, or with seine nets. These alternate collection methods were necessary because larger fish, such as older Atlantic croaker, were able to avoid an Otter trawl, or were found in untrawlable habitats such as shallow water, along marsh edges, and over oyster reefs. Fish liver tissue samples were analyzed for DNA-xenobiotic adducts in liver via Phosphorus 32-postlabeling assay to indicate genotoxic exposure.The analysis of DNA adducts by Phosphorus 32-postlabeling is a multistep process involving a series of biochemical reactions. First, DNA is hydrolyzed enzymatically to 3'monophosphates. The digest is then enriched in xenobiotic-modified mononucleotides by the selective removal of normal nucleotides. Following the enrichment step the adducted DNA is enzymatically labeled at the 5'-hydroxyl position with [Phosphorus 32] phosphate to form [5'-Phosphorus 32] deoxyribonucleoside 3',5'-bisphosphates. Separation of the Phosphorus 32-labeled adducts is usually accomplished by two-dimensional, thin-layer chromatography (TLC) on polyethyleneimine (PEI)-modified cellulose sheets. Autoradiography or storage phosphor imaging (Reichert et al., 1992) is then used to locate the radiolabeled adducts on the chromatogram (Reichert and French, 1994).The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples and analyzing standard reference materials.
Process_Date: 1991
Process_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization:
National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA)
Contact_Position:
National Status and Trends, Benthic Surveillance Project Manager
Contact_Address:
Address_Type: mailing and physical address
Address: 1305 East West Highway N/SCI1
City: Silver Spring
State_or_Province: MD
Postal_Code: 20910
Contact_Voice_Telephone: 30-713-3028
Contact_Facsimile_Telephone: 301-713-4388

Spatial_Data_Organization_Information:
Direct_Spatial_Reference_Method: Point

Spatial_Reference_Information:
Horizontal_Coordinate_System_Definition:
Geographic:
Latitude_Resolution: 0.0001
Longitude_Resolution: 0.0001
Geographic_Coordinate_Units: Decimal Degrees

Entity_and_Attribute_Information:
Detailed_Description:
Entity_Type:
Entity_Type_Label:
Dataset Parameters for Benthic Surveillance DNA Xenobiotic Adducts data
Entity_Type_Definition:
The BSdna_h data file reports information regarding DNA xenobiotic adducts study parameters.
Entity_Type_Definition_Source: National Status and Trends, Benthic Surveillance Program
Attribute:
Attribute_Label: NST_SITE
Attribute_Definition: 5 letter code site identifier.
Attribute_Definition_Source: NS&T, Benthic Surveillance Project
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name:
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts
Codeset_Source: National Status and Trends, Benthic Surveillance Project
Attribute:
Attribute_Label: YEAR
Attribute_Definition: Year the site was sampled.
Attribute_Definition_Source: NS&T, Benthic Surveillance Project
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name:
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts
Codeset_Source: National Status and Trends, Benthic Surveillance Project
Attribute:
Attribute_Label: SPEC_CD1
Attribute_Definition:
Species abbreviation, usually a 2 or 3 letter code which identifies the common species name. For example AF is used to define Artic Flounder.
Attribute_Definition_Source: NS&T, Benthic Surveillance Project
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name:
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts
Codeset_Source: National Status and Trends, Benthic Surveillance Project
Attribute:
Attribute_Label: REP
Attribute_Definition: Replicate sample, replicate of a unique sample.
Attribute_Definition_Source: NS&T, Benthic Surveillance Project
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name:
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts
Codeset_Source: National Status and Trends, Benthic Surveillance Project
Attribute:
Attribute_Label: SPEC_NO
Attribute_Definition: Laboratory specimen number.
Attribute_Definition_Source: NS&T, Benthic Surveillance Project
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name:
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts
Codeset_Source: National Status and Trends, Benthic Surveillance Project
Attribute:
Attribute_Label: OTHER_CD
Attribute_Definition:
Another site identifier, Codes used by NSandT participating laboratories.
Attribute_Definition_Source: NS&T, Benthic Surveillance Project
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name:
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts
Codeset_Source: National Status and Trends, Benthic Surveillance Project
Attribute:
Attribute_Label: PPL
Attribute_Definition: DNA Xenobiotic adducts. Phosphorus 32 post-labeling.
Attribute_Definition_Source: NS&T, Benthic Surveillance Project
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name:
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts
Codeset_Source: National Status and Trends, Benthic Surveillance Project
Attribute:
Attribute_Label: REV_DNA
Attribute_Definition: Last revision date of the DNA adducts file.
Attribute_Definition_Source: NS&T, Benthic Surveillance Project
Attribute_Domain_Values:
Codeset_Domain:
Codeset_Name:
Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts
Codeset_Source: National Status and Trends, Benthic Surveillance Project

Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization:
National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA)
Contact_Position:
National Status and Trends, Benthic Surveillance Project Manager
Contact_Address:
Address_Type: mailing and physical address
Address: 1305 East West Highway N/SCI1
City: Silver Spring
State_or_Province: MD
Postal_Code: 20910
Contact_Voice_Telephone: 301-713-3028
Contact_Facsimile_Telephone: 301-713-4388
Resource_Description: Downloadable Data
Distribution_Liability: None
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: ASCII
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name: <http://ccma.nos.noaa.gov/about/coast/nsandt/download.aspx>
Fees: None

Metadata_Reference_Information:
Metadata_Date: 20121220
Metadata_Review_Date: 20100521
Metadata_Contact:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization:
National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOAA), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA)
Contact_Position:
National Status and Trends, Benthic Surveillance Project Manager
Contact_Address:
Address_Type: mailing and physical address
Address: 1305 East West Highway N/SCI1
City: Silver Spring
State_or_Province: MD
Postal_Code: 20910
Contact_Voice_Telephone: 301-713-3028
Contact_Facsimile_Telephone: 301-713-4388
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: Local Time
Online_Linkage: <http://www.esri.com/metadata/esriprof80.html>


CoRIS:
CoRIS_ID: 20100521124441
CoRIS_Children: None
CoRIS_Beginning_Date: 19910101
CoRIS_Ending_Date: 19911231
CoRIS_Metadata_Link: <http://www.coris.noaa.gov/metadata/records/html/bsdna_h.html>
CoRIS_Tracking_ID: 4910

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