Identification_Information: Citation: Citation_Information: Originator: Department of Commerce (DOC), National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA) Publication_Date: 1992 Title: National Status and Trends, Benthic Surveillance Project DNA-Xenobiotic Adducts Data, 1991, National Centers for Coastal Ocean Science Geospatial_Data_Presentation_Form: table Publication_Information: Publication_Place: Silver Spring, MD Publisher: NOAA's Ocean Service, National Centers for Coastal Ocean Science (NCCOS) Online_Linkage: https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/ Description: Abstract: In order to determine the current status of and to detect any long-term trends in the environmental quality of U.S. nearshore waters, NOAA initiated the National Status and Trends program in 1984 with its National Benthic Surveillance Project. The primary objective of the Benthic Surveillance Project was to quantify concentrations of a suite of organic and inorganic contaminants in the livers of fish and surficial sediments from selected sites in the coastal and estuarine waters of the United States.In addition, the levels of certain indicators of the biological effects of these contaminants were measured. Incidences of visible lesions, including fin erosion, have been noted and histopathological examinations of various tissues have been carried out. Originally histopathological examinations determined the prevalence of any identifiable disease conditions in samples of liver, kidney, and gill tissue.In addition, the Benthic Surveillance Project tested fish livers from 30 locations in 1991 to identify the concentrations of DNA xenobiotic adducts. DNA xenobiotic adducts are a bioindicator of exposure to genotoxic compounds. Measurement of biological markers such as DNA xenobiotic adducts have been used to improve the evaluation of contaminant exposure and responses in indigenous fish, and thus reinforce the connection between exposure to toxic chemicals and the observed injuries. Purpose: In response to concerns over environmental quality of the Nation's coastal and estuarine ecosystems, NOAA created the National Status and Trends (NSandT) Program in 1984. From 1984 through 1993, the Benthic Surveillance Project monitored chemical concentrations in the livers (and for metabolites of PAH's in the bile) of bottom-dwelling fish and in sediments at the sites of fish capture. The Benthic Surveillance Project also measured the biological effects of contaminant exposure, primarily as prevalence's of toxicopathic liver diseases. Time_Period_of_Content: Time_Period_Information: Single_Date/Time: Calendar_Date: 1991 Currentness_Reference: publication date Status: Progress: Complete Maintenance_and_Update_Frequency: None planned Spatial_Domain: Bounding_Coordinates: West_Bounding_Coordinate: -166.5 East_Bounding_Coordinate: -67.3 North_Bounding_Coordinate: 70.5 South_Bounding_Coordinate: 25.6 Keywords: Theme: Theme_Keyword_Thesaurus: NCEI Geoportal Filter Theme_Keyword: CoRIS_Metadata Theme: Theme_Keyword_Thesaurus: CoRIS Discovery Thesaurus Theme_Keyword: Numeric Data Sets > Chemical Contaminants Theme: Theme_Keyword_Thesaurus: ISO 19115 Topic Category Theme_Keyword: environment Theme_Keyword: 007 Theme_Keyword: oceans Theme_Keyword: 014 Theme: Theme_Keyword_Thesaurus: CoRIS Theme Thesaurus Theme_Keyword: EARTH SCIENCE > Human Dimensions > Environmental Impacts > Contaminants Theme: Theme_Keyword_Thesaurus: None Theme_Keyword: DNA Theme_Keyword: deoxyribonucleic acid Theme_Keyword: xenobiotic deoxyribonucleic acid adducts Theme_Keyword: DNA xenobiotic adducts Theme_Keyword: DNA adducts Theme_Keyword: DNA abnormalities Theme_Keyword: genotoxic exposure Theme_Keyword: Coastal Monitoring Theme_Keyword: oceans Theme_Keyword: NSandT Theme_Keyword: NS&T Place: Place_Keyword_Thesaurus: None Place_Keyword: U.S. Exclusive Economic Zone Place_Keyword: Baltimore Harbor, Brewerton Channel Place_Keyword: Boston Harbor, Deer Island Place_Keyword: Cape Elizabeth, Richmond Island Place_Keyword: Cape Fear River, Horseshoe Shaol Place_Keyword: Charlotte Harbor, Cape Haze Place_Keyword: Chesapeake Bay, Chester River Place_Keyword: Chesapeake Bay, James River Place_Keyword: Chesapeake Bay, Patuxent River Place_Keyword: Choctawhatchee Bay, Choctawhatchee Bay Place_Keyword: Dana Point Harbor, Outside Place_Keyword: Delaware Bay, Cherry Island Range Place_Keyword: Johns Bay, Pemaquid Neck Place_Keyword: Lower Laguna Madre, Laguna Heights Place_Keyword: Lower Laguna Madre, Long Island Place_Keyword: New Bedford Harbor, Clarks Point Place_Keyword: Pascagoula River, Escatawpa River Place_Keyword: Pensacola Bay, Pensacola Bay Place_Keyword: Raritan Bay, East Reach Place_Keyword: Raritan Bay, Upper Bay Place_Keyword: Salem Harbor, Folger Point Place_Keyword: San Antonio Bay, Mosquito Point Place_Keyword: San Diego Bay, North Place_Keyword: San Diego Bay, Twenty Eighth Street Place_Keyword: San Francisco Bay, Hunters Point Place_Keyword: San Francisco Bay, Southampton Shoal Place_Keyword: San Pedro Bay, Outer Harbor Place_Keyword: Sapelo Sound, Dog Hammock Place_Keyword: St. Andrews Bay, Military Point Place_Keyword: St. Johns River, Arlington Channel Place_Keyword: St. Lucie River, Stuart Place: Place_Keyword_Thesaurus: CoRIS Place Thesaurus Place_Keyword: OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Place_Keyword: COUNTRY/TERRITORY > United States of America > Florida Place_Keyword: OCEAN BASIN > Atlantic Ocean Place_Keyword: OCEAN BASIN > Pacific Ocean Place: Place_Keyword_Thesaurus: CoRIS Region Place_Keyword: Florida Access_Constraints: None Use_Constraints: NOAA requests that all individuals who download NSandT data acknowledge the source of these data in any reports, papers, or presentations. If you publish these data, please include a statement similar to: "Some or all of the data described in this article were produced by NOAA through its National Status and Trends Program". Point_of_Contact: Contact_Information: Contact_Organization_Primary: Contact_Organization: National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA) Contact_Position: National Status and Trends, Benthic Surveillance Project Manager Contact_Address: Address_Type: mailing and physical address Address: 1305 East West Highway N/SCI1 City: Silver Spring State_or_Province: MD Postal_Code: 20910 Contact_Voice_Telephone: 301-713-3028 Contact_Facsimile_Telephone: 301-713-4388 Native_Data_Set_Environment: Microsoft Windows 2000 Version 5.0 (Build 2195) Service Pack 3; ESRI ArcCatalog 8.3.0.800 Data_Quality_Information: Logical_Consistency_Report: The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples and analyzing standard reference materials. To assess the reproducibility of results every tissue sample was analyzed in duplicate. Completeness_Report: The quality of the analytical data generated by the NSandT Program is overseen by the QA Project component, which has been in operation since 1985 and is designed to document sampling and analytical procedures, and to reduce intralaboratory and interlaboratory variation. The QA Project documentation will facilitate comparisons between different monitoring programs with similar QA activities and thus will extend the temporal and spatial scale of such programs. To document laboratory expertise, the QA Project requires all NSandT laboratories to participate in a continuing series of intercomparison exercises utilizing a variety of materials. The organic analytical intercomparison exercises are coordinated by the NIST, and the inorganic exercises by the National Research Council (NRC) of Canada. Details of quality assurance for the Benthic Surveillance Program can be found in Lauenstein and Cantillo, 1993. Positional_Accuracy: Horizontal_Positional_Accuracy: Horizontal_Positional_Accuracy_Report: The same kind of field/site data have been supplied since the start of the Benthic Surveillance Project, in 1984. Original site coordinates were derived from Loran-C time conversions. Early sites information resulting from Loran-C was converted from time delay information to latitudes and longitudes. These earlier data may be suspect when sites were located close to large structures that could have interfered with accurate time delays. When GPS was first available the signal was intentionally degrades so earlier coordinate information, even if it resulted from GPS, is not as accurate as data would be today.Because fish are not sessile, fish trawls have been made along different tracks in the water body of interest. The latitude/longitude coordinates provided in this file represent a nominal site center and trawling occurs within a 1 km radius of this location. Lineage: Process_Step: Process_Description: The primary collection apparatus was Otter trawls. Occasionally, fish were taken with hook and line, or with seine nets. These alternate collection methods were necessary because larger fish, such as older Atlantic croaker, were able to avoid an Otter trawl, or were found in untrawlable habitats such as shallow water, along marsh edges, and over oyster reefs. Fish liver tissue samples were analyzed for DNA-xenobiotic adducts in liver via Phosphorus 32-postlabeling assay to indicate genotoxic exposure.The analysis of DNA adducts by Phosphorus 32-postlabeling is a multistep process involving a series of biochemical reactions. First, DNA is hydrolyzed enzymatically to 3'monophosphates. The digest is then enriched in xenobiotic-modified mononucleotides by the selective removal of normal nucleotides. Following the enrichment step the adducted DNA is enzymatically labeled at the 5'-hydroxyl position with [Phosphorus 32] phosphate to form [5'-Phosphorus 32] deoxyribonucleoside 3',5'-bisphosphates. Separation of the Phosphorus 32-labeled adducts is usually accomplished by two-dimensional, thin-layer chromatography (TLC) on polyethyleneimine (PEI)-modified cellulose sheets. Autoradiography or storage phosphor imaging (Reichert et al., 1992) is then used to locate the radiolabeled adducts on the chromatogram (Reichert and French, 1994).The analytical instruments were calibrated by standard laboratory procedures including: constructing calibration curves, running blank and spiked quality control samples and analyzing standard reference materials. Process_Date: 1991 Process_Contact: Contact_Information: Contact_Organization_Primary: Contact_Organization: National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA) Contact_Position: National Status and Trends, Benthic Surveillance Project Manager Contact_Address: Address_Type: mailing and physical address Address: 1305 East West Highway N/SCI1 City: Silver Spring State_or_Province: MD Postal_Code: 20910 Contact_Voice_Telephone: 30-713-3028 Contact_Facsimile_Telephone: 301-713-4388 Spatial_Data_Organization_Information: Direct_Spatial_Reference_Method: Point Spatial_Reference_Information: Horizontal_Coordinate_System_Definition: Geographic: Latitude_Resolution: 0.0001 Longitude_Resolution: 0.0001 Geographic_Coordinate_Units: Decimal Degrees Entity_and_Attribute_Information: Detailed_Description: Entity_Type: Entity_Type_Label: Dataset Parameters for Benthic Surveillance DNA Xenobiotic Adducts data Entity_Type_Definition: The BSdna_h data file reports information regarding DNA xenobiotic adducts study parameters. Entity_Type_Definition_Source: National Status and Trends, Benthic Surveillance Program Attribute: Attribute_Label: NST_SITE Attribute_Definition: 5 letter code site identifier. Attribute_Definition_Source: NS&T, Benthic Surveillance Project Attribute_Domain_Values: Codeset_Domain: Codeset_Name: Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts Codeset_Source: National Status and Trends, Benthic Surveillance Project Attribute: Attribute_Label: YEAR Attribute_Definition: Year the site was sampled. Attribute_Definition_Source: NS&T, Benthic Surveillance Project Attribute_Domain_Values: Codeset_Domain: Codeset_Name: Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts Codeset_Source: National Status and Trends, Benthic Surveillance Project Attribute: Attribute_Label: SPEC_CD1 Attribute_Definition: Species abbreviation, usually a 2 or 3 letter code which identifies the common species name. For example AF is used to define Artic Flounder. Attribute_Definition_Source: NS&T, Benthic Surveillance Project Attribute_Domain_Values: Codeset_Domain: Codeset_Name: Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts Codeset_Source: National Status and Trends, Benthic Surveillance Project Attribute: Attribute_Label: REP Attribute_Definition: Replicate sample, replicate of a unique sample. Attribute_Definition_Source: NS&T, Benthic Surveillance Project Attribute_Domain_Values: Codeset_Domain: Codeset_Name: Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts Codeset_Source: National Status and Trends, Benthic Surveillance Project Attribute: Attribute_Label: SPEC_NO Attribute_Definition: Laboratory specimen number. Attribute_Definition_Source: NS&T, Benthic Surveillance Project Attribute_Domain_Values: Codeset_Domain: Codeset_Name: Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts Codeset_Source: National Status and Trends, Benthic Surveillance Project Attribute: Attribute_Label: OTHER_CD Attribute_Definition: Another site identifier, Codes used by NSandT participating laboratories. Attribute_Definition_Source: NS&T, Benthic Surveillance Project Attribute_Domain_Values: Codeset_Domain: Codeset_Name: Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts Codeset_Source: National Status and Trends, Benthic Surveillance Project Attribute: Attribute_Label: PPL Attribute_Definition: DNA Xenobiotic adducts. Phosphorus 32 post-labeling. Attribute_Definition_Source: NS&T, Benthic Surveillance Project Attribute_Domain_Values: Codeset_Domain: Codeset_Name: Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts Codeset_Source: National Status and Trends, Benthic Surveillance Project Attribute: Attribute_Label: REV_DNA Attribute_Definition: Last revision date of the DNA adducts file. Attribute_Definition_Source: NS&T, Benthic Surveillance Project Attribute_Domain_Values: Codeset_Domain: Codeset_Name: Data Set Parameters for Benthic Surveillance DNA Xenobiotic Adducts Codeset_Source: National Status and Trends, Benthic Surveillance Project Distribution_Information: Distributor: Contact_Information: Contact_Organization_Primary: Contact_Organization: National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA) Contact_Position: National Status and Trends, Benthic Surveillance Project Manager Contact_Address: Address_Type: mailing and physical address Address: 1305 East West Highway N/SCI1 City: Silver Spring State_or_Province: MD Postal_Code: 20910 Contact_Voice_Telephone: 301-713-3028 Contact_Facsimile_Telephone: 301-713-4388 Resource_Description: Downloadable Data Distribution_Liability: None Standard_Order_Process: Digital_Form: Digital_Transfer_Information: Format_Name: ASCII Digital_Transfer_Option: Online_Option: Computer_Contact_Information: Network_Address: Network_Resource_Name: https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/ Fees: None Metadata_Reference_Information: Metadata_Date: 20200329 Metadata_Review_Date: 20100521 Metadata_Contact: Contact_Information: Contact_Organization_Primary: Contact_Organization: National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOAA), National Centers for Coastal Ocean Science (NCCOS), Center for Coastal Monitoring and Assessment (CCMA) Contact_Position: National Status and Trends, Benthic Surveillance Project Manager Contact_Address: Address_Type: mailing and physical address Address: 1305 East West Highway N/SCI1 City: Silver Spring State_or_Province: MD Postal_Code: 20910 Contact_Voice_Telephone: 301-713-3028 Contact_Facsimile_Telephone: 301-713-4388 Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata Metadata_Standard_Version: FGDC-STD-001-1998 Metadata_Time_Convention: Local Time Online_Linkage: http://www.esri.com/metadata/esriprof80.html ESRI Metadata Profile CoRIS: CoRIS_ID: 20100521124441 CoRIS_Children: None CoRIS_Beginning_Date: 19910101 CoRIS_Ending_Date: 19911231 CoRIS_Metadata_Link: https://www.coris.noaa.gov/metadata/records/html/bsdna_h.html CoRIS_Tracking_ID: 4910